Because citron kinase is localized in cleavage furrows, citron kinase may be involved in regulating di-phosphorylation of MLC during cytokinesis. Although both mono- and di-phosphorylated MLC were found in cleavage furrows, di-phosphorylated MLC showed more constrained localization than did mono-phosphorylated MLC.

These observations suggest that citron kinase phosphorylates MLC to generate di-phosphorylated MLC in vivo. The expression of full-length citron kinase also increased di-phosphorylation during cytokinesis. Furthermore, the kinase domain was able to increase di-phosphorylation and restore stress fiber assembly even when ROCK was inhibited with a specific inhibitor, Y-27632. We found that the expression of the kinase domain of citron kinase resulted in an increase in MLC di-phosphorylation. Unlike ROCK, however, citron kinase did not phosphorylate the myosin binding subunit of myosin phosphatase, indicating that it does not inhibit myosin phosphatase.

About the Author: Fumio Sasakiis the co-editor-in-chief at Wani Books and lives in a 215-square-foot apartment in Tokyo, furnished with a small wooden box, a desk, and a roll-up futon pad. 'synopsis' may belong to another edition of this title. We found that citron kinase phosphorylated regulatory light chain (MLC) of myosin II at both Ser-19 and Thr-18 in vitro. Sasaki explores the philosophy and cultural history of minimalism from Buddhism to Steve Jobs. However, no substrates are known for citron kinase. Both ROCK and citron kinase are suggested to play a role in cytokinesis. He explains why ‘This is what the place looks like. After deciding to scorn possessions, he began feeling happier. Citron kinase is a Rho-effector protein kinase that is related to Rho-associated kinases of ROCK/ROK/Rho-kinase family. Fumio Sasaki owns a roll-up mattress, three shirts and four pairs of socks.

Sasaki fumio minimalist